Department of Microbiology

Cell Culture Technique workshop

Department of Microbiology organized a workshop at Central Facilities For Research & Development, Osmania University. 35 students along with two faculty Dr.K. Anuradha and Dr. Y.Aparna attended the workshop on 16th March 2024 cell culture technique provided the participants with hands-on experience and theoretical knowledge regarding the principles and practices of cell culture. The event focused on various aspects including cell culture setup, maintenance, and troubleshooting.The session began with an overview of cell culture, emphasising its importance in various fields such as medicine, biotechnology, and research by Prof. Sandeepta Burgula followed by a lecture on cancer cell signalling- molecular tools and methods by Dr. Raghu Gogada Participants were introduced to sterile techniques essential for maintaining a contamination-free cell culture environment. Handling of equipment such as Biosafety cabinet (level II - V), Incubation facilities, Refrigerators, media components ,inverted microscope ,Tissue culture, reagents, and surfaces were demonstrated. The process of preparing cell culture media, including the selection of appropriate media [DMEM,RPMI 1640,MEM] addition of supplements [ serum, antibiotics, bicarbonate buffers, CO2 ] and adjustment of pH, was demonstrated step-by-step.Participants learned the technique of cell passaging, which involves the transfer of cells from one culture vessel to another. The importance of maintaining cell viability and preventing contamination during passaging was highlighted.Methods for cell counting and viability assessment, such as hemocytometer counting and trypan blue exclusion, were demonstrated. These techniques are crucial for determining cell concentration and assessing cell health. The cell culture technique demonstration provided participants with a comprehensive understanding of the principles and practices involved in cell culture.

National Science Day

The Department of Life Sciences, BVC celebrated National Science day-2024 on 28th February, 2024. The theme of the programme was “INDIGENOUS TECHNOLOGIES FOR VIKSIT BHARAT”. The celebrations started with an inaugural programme, the principal Dr.GSVRK Choudhary and heads of all Life Science Departments interacted with the students and explained to them the importance of National Science Day. The Chief Guest for the inaugural programme, Dr.Rama Krishna Kancha, Director,Prof.G.M Reddy’s Centre for Plant Molecular Biology, Osmania University, Hyderabad delivered the keynote address where he stressed on the importance and significance of Science in daily life. He also elaborated on how science has improved human health by citing the examples of recombinant insulin and thyroid treatment. The programme had a participation of about 150 students. Two events; JAM-Just A Minute and Essay writing competitions for students were conducted post inaugural. 17 students participated in JAM which was judged by Dr. K. Anuradha, Head, Department of Microbiology and Dr.A.Sai Padma, Head, Department of Biochemistry & Nutrition. The topic for Essay writing competition was “Synergizing AI with Indigenous Technologies for Sustainable Development in Viksit Bharat”.15 students participated in the competition which was judged by Dr. B. Kalpana, Head, and Department of Genetics & Biotechnology.Mrs. Mary Nygi Kurian, Head, Department of Chemistry. In the afternoon session, e-Poster competition was conducted department-wise. The students presented e-poster on the project work carried out by them in the college. 7 teams participated from Department of Biochemistry and Nutrition. 6 teams participated from the Department of Microbiology.7 teams participated from the Department of Chemistry.13 teams participated from the Department of Genetics and Biotechnology. The e-posters were judged by the Life Sciences PG faculty members. About 120 students of Semester VI of UG Life Sciences participated in the e-poster competition. The National Science Day celebration provided an excellent platform for students to express their innovative ideas and creativity in all the areas of Life Sciences.

Industrial Trip to Crown Beverages

On 24th February 2024, the Department of Microbiology organized an industrial trip to Crown Beverages for 37 M.Sc. Microbiology Final year students. The purpose of the visit was to provide students with practical exposure to the industrial processes involved in the production of beer. Dr. S. Anju and Dr. Deepak Raj accompanied the students to the industry.Upon arrival, the students were warmly welcomed by the staff of Crown Beverages. The visit commenced with a detailed briefing about the beer fermentation process, economics involved, and the various important equipment used in the industry. The session was led by the chief in charge, who provided comprehensive insights into the intricacies of beer production.Following the briefing session, the students were divided into groups and taken on a guided tour of the factory. The tour encompassed various sections of the facility, including packaging sections, fermentation units, quality control labs, and raw material processing units. At each station, experts from Crown Beverages demonstrated the processes involved in beer production, allowing students to gain a firsthand understanding of the operations.The industrial trip provided the students with an elaborate exposure to the processes involved in the production of beer. They witnessed each step of the process, from raw material processing to packaging, and gained valuable insights into the quality control measures implemented by the industry. Moreover, interactions with industry professionals enabled students to deepen their understanding of theoretical concepts learned in the classroom.The visit enabled students to translate theoretical knowledge into practical understanding by observing real-world industrial processes.Students learned about the importance of quality control measures in ensuring the consistency and safety of beer production.Interactions with industry professionals shed light on the economic and operational dynamics of the brewing industry.The guided tour fostered teamwork and collaboration among students, encouraging them to exchange ideas and insights.

Training Program

The Department of Microbiology organised a training program on ‘Cell Culturing Techniques’ in association with Centre Facilities for Research & Development, CFRD, Osmania University on 24th Feb 2024 for BSc Final year students under DBT Star College Scheme. About 10 students from MGC and MNDC accompanied by the faculty Dr T Chaitanya attended the program. The first lecture was delivered by Prof. Sandeepta, Head, Department of Microbiology and Director, CFRD, OU. where she gave an introduction of Animal cell culture about the history of tissue/cell culture. This lecture also covered topics like media required for cell culturing, precautions, and safety measures to be taken and monoclonal cells. Importance and applications of cell culturing was also discussed. The second lecture was given by Dr Raghu Gogada, Scigene Bio Pvt., Ltd., M2- Technology Hub, IDA Uppal.He briefed the students about apoptosis cancer signalling. He explained about different types of cell death which includes type l -Apoptotic cell death, type ll - Autophagic cell death and type lll - Necrotic cell death. He focused on Apoptotic cell death mediated by mitochondria and the role of resveratrol in cancer signalling. The students were made aware of different apoptosis detection techniques like microscopy, immunolabelling, western blotting, immunoprecipitation, western blotting and flow cytometry. The detailed explanation of the significance of resveratrol in induction of caspase dependent apoptosis and through the statistical data. Later on, visited the cell culturing laboratory explained how safety precautions will be taken and sterility is maintained and also observed the adherent and suspended cells under the inverted microscope and counted the number of cells using trypan blue staining.

Workshop on PCR

The Two – Day Workshop on the theme “Molecular Identification of Microbial Cultures” was a collaborative endeavour between the Department of Microbiology, BVC and Bioserve Biotechnologies (India) Pvt. Ltd. The workshop aimed to provide a hands – on experience to students about PCR and Gel Electrophoresis Techniques and in – depth understanding on the working principles of Sanger’s Sequencing. The program unfolded through four meticulously curated sessions, Dr. Bhawna Dubey (Chief Scientific Officer) enriched the inaugural session of the programme. She gave theoretical insights on how Polymerase Chain Reactions work and the concepts behind it. After a short break, the students were divided into two batches and were taken to the laboratories (Watson Lab & Kary Mullis Lab) for a demo setup followed by hands – on training on PCR. Ms. Nikki, Dr. Rosy, Mr. Siva, Mr. Manoj & Ms. Preeti guided the students on the optimised protocol.One batch worked with Bacterial sample and the other worked with Fungal sample. After the students setup their own PCRs, the PCR was run.

Outreach Activity Report: Advances in Research and Career Opportunities in Life Sciences

The outreach activity organized for M.Sc. Microbiology final year students on February 19th, 2024 at School of Life Sciences, Hyderabad Central University, Gachibowli, Hyderabad; aimed to provide insights into recent advancements in research and career opportunities within the field of life sciences. The event featured esteemed speakers from diverse backgrounds, including academia, research institutions, industry, and science communication. Speakers and Topics: Dr. Manish Jaiswal, Scientist at TIFR: Topic: "Unveiling the Structure of Mitochondria" Dr. Jaiswal delved into the intricate details of mitochondrial structure, shedding light on recent breakthroughs and their implications for understanding cellular function and disease mechanisms. His talk provided a comprehensive overview of the latest advancements in mitochondrial research. Dr. Nagendra Hegde, NIAB: Topic: "Exploring Career Opportunities in Life Sciences" Dr. Hegde offered valuable insights into the diverse career paths available in the field of life sciences. He discussed various opportunities in academia, industry, research institutions, and beyond, emphasizing the importance of interdisciplinary skills and lifelong learning in navigating a successful career in the life sciences. Dr. Jagdeesh Gandla, COO at FABA: Topic: "Industry Perspectives on Research and Innovation" Dr. Gandla shared his expertise on bridging the gap between academia and industry in driving innovation within the life sciences sector. He highlighted the role of biotech companies in translating research findings into practical applications, offering valuable insights into industry trends and challenges. Mrunal Kulkarni, Freelance Sci-Artist: Topic: "Communicating Science Through Art" Ms. Kulkarni showcased the power of visual storytelling in communicating complex scientific concepts to a broader audience. Through her captivating artwork, she demonstrated how art can serve as a bridge between science and society, fostering engagement, curiosity, and understanding. Interactive Q&A sessions after each talk facilitated meaningful discussions and allowed students to clarify doubts and seek further insights from the speakers. Networking opportunities enabled students to connect with professionals from different sectors of the life sciences community, fostering potential collaborations and mentorship relationships. The event concluded with a panel discussion, where speakers addressed emerging trends, challenges, and future prospects in the field of life sciences, inspiring students to embark on their professional journeys with confidence and enthusiasm. The outreach activity proved to be a resounding success, providing M.Sc. Microbiology final year students with valuable knowledge, inspiration, and networking opportunities. By bringing together experts from academia, industry, and science communication, the event served as a platform for fostering interdisciplinary collaboration and empowering the next generation of life scientists to make meaningful contributions to society.

National Seminar

A two-day National Seminar on 'The Microbiome Revolution: From Basic Science to Therapeutic Innovations' took place on January 24th and 25th, 2024 was organized by Department of Microbiology at Bhavan’s Vivekananda College of Science, Humanities and Commerce under the DBT Star College Scheme, in collaboration with the Microbiologist Society of India. Inaugural ceremony was presided by Chief Guest Prof. B. Sesikeran, Former Director NIN-ICMR, Hyderabad, and Guest of Honor Prof. Gita Sharma along with Vice Chairman Air Commodore Sri. JLN Sastry VSM BVB, Principal Dr. G.S.V.R. K Choudary, Convener Dr. K. Anuradha and other esteemed guests. The event attracted participation from prestigious institutions including Little Flower Degree College, Sri Sathya Sai Institute of Higher Learning, Sree Vani Women’s College, Hyderabad, Aurora’s Degree & PG College active involvement from Department of Microbiology and Biochemistry at Bhavan’s Vivekananda College. The seminar drew a total of 200 participants who actively engaged in the enlightening sessions.

Skill Training Program

On December 21st and 22nd 2023, a total of 30 students from the 3rd year of MBGC, MNDC programs visited Accu Analytical, a pharmaceutical lab, for an insightful skill training program. This training program was organized by the Department of Microbiology, BVC under DBT Star College scheme.Accu Analytical, a prominent lab in the food testing services and pharma analytical lab and NMR Analytical services provided students with a comprehensive understanding of analytical testing of chemical compounds like HPLC, NMR and GC

Guest Lecture

On December 12, 2023, the Department of Microbiology at BVC organized a Guest Lecture as part of the DBT STAR COLLEGE Scheme. The esteemed guest speaker for the event was Dr. Hameeda Bee, Associate Professor and Chairperson of the Board of Studies at the Department of Microbiology, Osmania University. The Lecture focused on the intriguing topic of "Human Microbiome," drawing the active participation of 132 students from both undergraduate and postgraduate Microbiology programs. Dr. Hameeda Bee initiated the talk by highlighting the significance of delving into subjects beyond the scope of examinations. She skillfully differentiated microorganisms into categories of "Goodies and Baddies," shedding light on their diverse roles. Emphasizing the fusion of art and science, she encouraged students to view microbiology from a holistic perspective. The Lecture also delved into advanced techniques, including Culture-dependent and Culture-independent methods such as Metagenomics. Dr. Hameeda Bee took the audience on a journey through the intricacies of microbiota at various levels, ultimately connecting it to the realm of Artificial Intelligence (AI). The talk not only enriched the students' understanding of the Human Microbiome but also underscored the importance of interdisciplinary approaches in scientific exploration.

Industrial Visit

On December 7, 2023, a total of 75 students from the 3rd year of the MBGC, MNDC, and MBIC programs visited Crown Beverages and Charminar Beverages for an insightful industrial field trip. This industrial visit was organised by Department of Microbiology, BVC under DBT Star college scheme. Faculty members, Dr K.Anuradha, Dr Y. Aparna, DrS.Shalini Devi, and Dr Mahalakshmi accompanied the students. The visit aimed to bridge theoretical knowledge and practical insights. Crown and Charminar Beverages, a prominent player in the beverage industry, provided students with a comprehensive understanding of the beer manufacturing process. The tour covered each stage, from raw material selection to packaging, offering a tangible link between classroom teachings and real-world applications. Students engaged in interactive sessions with technical experts, discussing brewing techniques, quality control measures, and the precision involved in the manufacturing process. Faculty guidance facilitated meaningful discussions, ensuring students gained maximum insights from the trip. The industrial field trip to Crown Beverages proved invaluable, enhancing academic growth and instilling appreciation for the complexities of the beverage industry.

Guest Lecture

A guest lecture was organised by department of Microbiology, BVC, under DBT STAR COLLEGE Scheme on 2-12-2023 in online mode. Guest speaker, Dr. Gnanesh Nanjappa, Professor and Dean, Department of Genetics and Plant Breeding. Sampurna International Institute of Agri Science & Horticultural Technology, Karnataka, INDIA explained his research expertise on the topic “Occurrence, molecular characterization and standardization of screening techniques of fungal pathogens: Current Trends and Future Perspectives”. Students of UG and PG microbiology(120 students) took active participation. Various aspects of isolation, identification of fungal pathogens, Importance of multilocus sequencing analysis in fungal molecular characterization were well explained in this lecture.

Guest Lecture

Dr. Subha Narayan Das, Assistant Professor, Department of Botany, Indira Gandhi National Tribal University (IGNTU), Lalpur, Amarkantak - 484887, Madhya Pradesh, India delivered an online guest lecture entitled “Plant Immunity – How Plants Defend Themselves” to the postgraduate students of Department of Microbiology, Bhavan’s Vivekananda College, Sainikpuri, Hyderabad, on November 24, 2023, from 11.00 AM to 1.00 PM. The link for the Google meeting was: https://meet.google.com/iqj-mxyc-ovk. Dr. Das’s talk focused on three themes: 1, How plant pathogens attack plants; 2, How plants defend themselves; and 3, How biochemicals can be used to prime plants, one of the strategies to protect crops from plant pathogens. Dr. Das’s lecture helped students understand the topic more effectively and learn about the relevant research performed by Dr. Das and his associates in the area of ‘crop protection’. After the lecture, a few students interacted with Dr. Das and expressed their happiness for attending the motivating lecture on Plant-Pathogen Interactions.

Visit to IICT

M.Sc Final students of Microbiology and Biochemistry Departments had attended AYURVEDA DAY –Ayurveda for one Health on 8th November at IICT. This programme was organized by IICT .an CSIR institute in collaboration with Ministry of AYUSH. Bhavan’s college had received invitation for the one day programme from IICT, under the MOU signed by both the organizations. Dr.J.Sarada from Microbiology and Dr.Manju Devi from Biochemistry departments had accompanied the students on 8th November 2023. Total 50 students from both the departments had participated in the event. Programme started with the welcome address by Dr. D.Srinivas Reddy ,Director CSIR-IICT , who briefed about the programme and the occasion followed by the Chief guest Address - Dr.Ramachandra from Naturopathy ,Siddhartha Yoga vidyalayam, Nelakondapalli ,Khammam. He discussed on the importance of AYURVEDA in present scenario in treating various diseases and requested the ministry of AYUSH for research on unexplored Phytochemicals. Later Guest of Honour, Padmasri Dr.M.A.Waheed, Unani expert and practitioner spoke on the occasion about the significance of AYUSH initiatives and Pharmacodynamics and upcoming research areas. Dr. G. Penchala Prasad, Asst.Director, National Institute on Indian medical Heritage, Hyderabad spoke about vriksha ayurveda and pasuayurveda along with Rasayana concept and personalized medicine and different practices in medicine. Programme concluded with the lecture on Safety , Efficacy and toxicity of phytochemicals by Dr. K.Nagaiah, chief scientist ,IICT .

Industrial Visit

Students of MSc Microbiology final year from Department of Microbiology along with faculty Dr. Anuradha, Dr. Y.Aparna visited Navratna Crop Science Pvt Ltd at IDA Cherlapally Medchal, Malkajgiri, Secunderabad, Telangana on 4th October from 10.00 AM to 4.00 PM This visit mainly focussed on to understand the procedures involved in biofertilizer production , the technology and the equipment used. Dr.Sarita Reddy explained about the facilities available inside the Industry, their methods of collecting raw materials, the Equipment used for the extraction of ecofriendly biofertilizers and their storage unit. Visit started with a presentation by Dr. Sarita Reddy, explaining different types of microorganisms (fungi, yeasts, bacteria) & equipment(fermenter) used in production process. The unit used cheaper raw materials (rice grist) as substrate for cultivating microorganisms in large scale. The fermenters used, play a vital role in the production of biofertilizers, biopesticides, and other agricultural inputs, contributing to sustainable farming practices and environmentally friendly solutions. Other than biofertilizers organic fertilisers, macronutrient (foliar spray) formulations, micronutrient (soil application) formulations, water soluble fertilisers, biostimulants, water soluble fertilisers etc were produced at ondustry on a large scale. The visit ended with demonstrating the packaging of products and their storage.

Microbial Marvels

Department of microbiology on September 23rd ,2023 in collaboration with the Microbiologists society of India and BVC student’s unit, conducted an exhibition on microbial products in honor of World Microorganisms Day. Various exhibits were put forth and their applications and uses were explained to the students of Bhavans Sri Ramakrishna Vidyalaya and Bhavans Aurobindo Junior College. Nearly, 350 students attended the exhibit and had the opportunity to get insights on the same. The exhibits showcased included that of-- Vedic Microbiology, Probiotics, Liquid 3, Industrial Fermenter, Soil and agriculture Microbiology, Human Microbiome, Vaccine Technology, Exo Microbiology, Role of Microorganisms in different stages of life and Antibiotic production. Honourable guest Dr. Kiran from Biological E Pvt.Ltd., judged the exhibits and complemented everyone. The exhibit “Liquid 3” bagged the 1st place; followed by “Exomicrobiology” and “Vedic microbiology” which bagged the 2nd and 3rd positions respectively. Students attended a quiz at the end based on the exhibits that were shown and information presented to them. Feedback forms were given to the students to know the areas of improvement. Surprisingly, it was found that more such events need to be conducted so as to attain more insights on this emerging field of science.